the effect of mg2+ and mn2+ on over-production of interleukin-2 in recombinant e.coli
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abstract
in order to increase the productivity of human interleukin-2 (il-2) as a model protein in the recombinant e.colibl21 (de3), the effect of magnesium acetate and manganese acetate was studied based on full factorial design experiments magnesium acetate and manganese acetate and their interaction exhibited an increasing effect on il-2 expression level upto 25.24%, 16.80% and 23.30%, respectively, in comparison to 10.76% for the standard m9 medium(as usual defined medium in high cell density culture). magnesium acetate was the most effective salt.other experiments were performed to investigate the effect of magnesium and acetate ions separately. results revealed that m9 medium with 1.5 times of magnesium ion concentration resulted in the highest expression level.
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Journal title:
iranian journal of chemistry and chemical engineering (ijcce)Publisher: iranian institute of research and development in chemical industries (irdci)-acecr
ISSN 1021-9986
volume 32
issue 1 2013
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